NOAA - Great Lakes Environmental Research Laboratory
| Transect Line | Basin | Latitude | Longitude |
| SW5-NNE East | West | 41 39.777 | 82 32.905 |
| SW5-NNE West | West, West of North and South Bass Islands | 41 31.059 | 82 32.905 |
| I East | Central (West End) | 41 43.027 | 82 11.047 |
| I West | Central (West End) | 41 40.181 | 82 28.544 |
| H East | Central | 41 50.07 | 82 11.994 |
| H West | Central | 41 49.670 | 82 23.16 |
| A North | Central | 42 14.90 | 82 4.00 |
| A South | Central | 41 30.80 | 81 43.50 |
| B North | Central (South Shore, by Conneaut) | 42 16.60 | 81 50.00 |
| B South | Central | 41 37.45 | 81 32.00 |
| C North | Central | 42 33.90 | 81 28.30 |
| C South | Central | 41 49.10 | 81 9.40 |
| D North | Central | 42 38.3 | 81 1.00 |
| D South | Central | 41 56.3 | 80 43.80 |
